Polymerase Chain Reaction - or PCR - is known as one of the most ubiquitous and powerful techniques in life science laboratories. PCR is a highly sensitive and selective method to produce many copies of a particular piece of DNA. This pathway details applications of PCR, its mechanism and primer design, and demonstrates how the method is used in the lab.

This interactive introduces how mistakes in the genome can cause disease. The gene editing tool CRISPR-Cas9 can correct these mistakes and potentially cure disease.

Este interactivo desplegable muestra el motivo por el cual se necesita una técnica como la electroforesis en gel para ordenar moléculas de ADN de distinto tamaño, incluidos los plásmidos de ADN lineal y de ADN circular. El interactivo también analiza el motivo por el cual no siempre es posible determinar el tamaño de un trozo circular de ADN a partir de la distancia que recorre sobre un gel.

This scrollable interactive shows why a technique like gel electrophoresis is needed to sort different size DNA molecules, including both linear DNA and circular DNA plasmids. The interactive also discusses why it is not always possible to judge the size of a circular piece of DNA from the distance it runs on a gel.

This scrollable interactive takes users on a tour of the DNA double helix, explaining how nucleotide base pairing leads to DNA's unique shape. Gel electrophoresis is also introduced as a technique that can help sort and estimate the size of both linear and circular DNA molecules.

This pathway introduces you to the virtual lab simulations available on LabXchange, and how these simulations can be used to teach lab skills like gel electrophoresis.

This pathway introduces gel electrophoresis, a technique used to separate biological molecules. This pathway also supports ABE lab 1.2: Gel Electrophoresis.

This pathway demonstrates how a fundamental tool in biotechnology, DNA gel electrophoresis, can be used to verify successful production of a recombinant plasmid. This pathway supports ABE labs 4 and 4a.