このシミュレーションは、バーチャルラボ環境でライゲーション反応の実施を練習する機会を提供します。このプロセスでは DNA リガーゼという酵素を使い、制限酵素消化で得られる相補的な平滑末端または粘着末端を用いて DNA 断片 2 つをつなげます。
이 시뮬레이션은 가상 실험실 환경에서 리게이션 반응 실험을 실습할 수 있는
기회를 제공합니다. 이 과정에서는 제한효소 절단으로부터 생성된 상보적인
평활 말단 또는 점착성 말단을 사용하여 2개의 DNA 단편을 부착하기 위해
효소인 DNA 리가아제를 사용합니다.
As a trained physician and historian of medicine, David Jones is uniquely positioned to comment on the COVID-19 pandemic. In this episode of the Xchange, we discuss the social dimensions of disease, the history of epidemics in America, and what lessons we have - and haven’t - learned from our past. A new virtual interview series from LabXchange at Harvard University, the Xchange showcases the diverse roles, projects, and initiatives that make up the intersecting fields of science, education, and health. Hosted by LabXchange Faculty Director Robert Lue, our first season features a range of perspectives on the impact of COVID-19.
David Reese is executive vice president of research and development for the Amgen Corporation. On this episode of The Xchange, David helps demystify the process of drug development, discusses the unique challenges that COVID-19 presents, and shares his thoughts on STEM career paths today. Join us for “From a Molecule to a Medicine” on the Xchange. A new virtual interview series from LabXchange at Harvard University, the Xchange showcases the diverse roles, projects, and initiatives that make up the intersecting fields of science, education, and health. Hosted by LabXchange Faculty Director Robert Lue, our first season features a range of perspectives on the impact of COVID-19.
This video explains the different ways the gene editing complex CRISPR-Cas9 is delivered into cells.
This lab video demonstrates how to run a typical protein gel in detail. This method is also called SDS-PAGE and separates proteins by molecular weight.
This pathway introduces the design of guide RNAs to disrupt a macrophage gene with the gene editing tool CRISPR-Cas9. Afterwards, a DNA cleavage assay is created to test how well the designed gRNAs work.
This pathway explores the concepts of diffusion and osmosis and the factors, like size, that impact the ability of molecules to diffuse across a membrane. There are several opportunities throughout the pathway to experiment with these concepts through simulations.
Met deze simulatie kunt u een restrictiedigestie oefenen in een virtuele labomgeving. Restrictie-enzymen worden gebruikt om de DNA-strengen van twee plasmiden te knippen; dit levert DNA-fragmenten op met complementaire kleverige uiteinden, die opnieuw kunnen worden samengevoegd om een recombinant plasmide te creëren.
In questo video, Monique dimostra come eseguire una digestione degli enzimi di restrizione in laboratorio.
In questo video, Monique dimostra come eseguire una digestione degli enzimi di restrizione in laboratorio.
Questa simulazione offre l’opportunità di praticare una digestione da restrizione in un laboratorio virtuale. Gli enzimi di restrizione vengono utilizzati per tagliare i filamenti di DNA di due plasmidi, per produrre frammenti di DNA con estremità coesive complementari che possono essere riassemblati per creare un plasmide ricombinante.
Cette simulation permet de s’entraîner à réaliser une digestion par restriction dans un environnement de laboratoire virtuel. Les enzymes de restriction sont utilisés pour couper les brins d’ADN de deux plasmides, en produisant des fragments d’ADN dotés d’extrémités collantes complémentaires pouvant être réassemblées pour créer un plasmide recombinant.
En este video, Monique demuestra cómo realizar una digestión con enzimas de restricción en el laboratorio.
Esta simulación ofrece la oportunidad de practicar una digestión de restricción en un laboratorio virtual. Las enzimas de restricción se utilizan para cortar las cadenas de ADN de dos plásmidos, lo que produce fragmentos de ADN con extremos cohesivos complementarios que pueden volver a ensamblarse para crear un plásmido recombinante.
Esta simulação proporciona uma oportunidade de praticar uma digestão por enzimas de restrição em um cenário de laboratório virtual. As enzimas de restrição são usadas para cortar as cadeias de DNA de dois plasmídeos, produzindo fragmentos de DNA com extremidades complementares coesivas que podem ser remontadas para criar um plasmídeo recombinante.
On LabXchange, you can find free online textbooks that are dynamic and continually updated. We make digital textbooks from respected sources more flexible. Individual sections of a textbook can be combined with other interactive content. This helps you understand different contexts and share new narratives.
Bu simülasyonda, rekombinant plazmid ile dönüştürülmüş bakteri
hücrelerinin nasıl çoğaltıldığını incelemek ve ilgilenilen genin
ekspresyonunu değerlendirmek için seçici besiyeri kullanılmaktadır.
This pathway explores the importance of biodiversity and human impact on the environment.
This video introduces the features of LabXchange designed to support educators in creating customized learning experiences.